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Thursday 27 July 2006

Enhancement of serotonin- and bradykinin-evoked calcium ion dynamics in

By: Imanishi T, Matsushima K, Kawaguchi A, Wada T, Yoshida S, Ichida S

Dynamic changes in the concentration of intracellular free-calcium ion ([Ca(2+)](i)) by carbachol (CCh) and neurotransmitter candidates was investigated in undifferentiated and differentiated neuroblastomaxglioma hybrid NG108-15 (NG) cells.

[Ca(2+)](i) was increased in a dose-dependent manner by bradykinin (BK) and serotonin (5-HT) in differentiated NG cells, and the response to BK and 5-HT was significantly greater than that in undifferentiated NG cells. The EC(50) value of BK was approximately 1.5x10(-8)M in both undifferentiated and differentiated NG cells.

The EC(50) value of 5-HT in differentiated NG cells was about 5x10(-6)M. The response to BK and 5-HT was almost completely inhibited by 10nM Hoe140 (a BK B2 receptor antagonist) and 3nM tropisetron (a 5-HT(3) receptor antagonist), respectively. These results suggest that there are some mechanisms by which the response evoked by BK and 5-HT is up-regulated in differentiated NG

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